TRBC1 antibody (APC)

• Catalog Number: orb669274
• Category: Antibodies
• Description: Mouse monoclonal antibody against TRBC1 conjugated to APC
• Clonality: Monoclonal
• Clone Number: JOVI.1
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG2a kappa
• Immunogen: thymus, spleen, and mesenteric lymph nodes isolated from a mouse transgenic for human TCR Vbeta3Cbeta1
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: APC
• Target: TCR Cbeta1
• Entrez: 28639
• UniProt ID: P01850
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-TCRCB1 antibody, anti-TRBC1 antibody
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human TCR Cbeta1 (JOVI.1) APC antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD3(UCHT1) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).

Separation of human TCR Cbeta1 positive lymphocytes (red-filled) from TCR Cbeta1 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human TCR Cbeta1 (JOVI.1) APC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR Cbeta1 (JOVI.1) APC antibody (4 μl reagent / 100 μl of peripheral whole blood).