Transcriptional regulator ERG Antibody / ERG

• Catalog Number: orb2635668
• Category: Antibodies
• Description: ERG (ETS-related gene) is a proto-oncogene, a member of the ETS family of transcription factors. The ERG gene encodes for a nuclear protein, also called ERG, which is involved in hematopoietic and endothelial development. ERG remains constitually expressed in endothelial cells in blood and lymphatic vessels, and in bone marrow stem cells. ERG is expressed in virtually all endothelial neoplasms including hemangioendothelioma, angiosarcoma and Kaposi sarcoma. ERG is overexpressed secondary to gene rearrangement in cases of prostate adenocarcinoma, gastrointestinal stromal tumor, synovial sarcoma, meningioma, epithelioid sarcoma, malignant rhabdoid tumor, acute myeloid leukemia and blastic extramedullary myeloid tumor, and rarely Ewing sarcoma / primitive peripheral neuroectodermal tumor, chondrosarcoma, osteosarcoma, and rhabdomyosarcoma. For the identification of endothelial differentiation ERG seems more sensitive and specific than any other marker. Moreover, the interpretation is often easier due to the nuclear reaction, which also allows for double stains with cytoplasmic markers like podoplanin. Among carcinomas, ERG is highly specific for prostate, while the sensitivity is moderate.
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 450 to 479 was used as the immunogen for the Transcriptional regulator ERG antibody.
• UniProt ID: P11308
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the Transcriptional regulator ERG antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: rERG/6843
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Transcriptional regulator ERG antibody is available for research use only.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

SDS-PAGE analysis of purified, BSA-free Transcriptional regulator ERG antibody (clone rERG/6843) as confirmation of integrity and purity.

Left: IHC staining of FFPE human prostate tissue tissue with Transcriptional regulator ERG antibody (clone rERG/6843) at 2 ug/ml. Right: Negative control staining of FFPE human brain tissue using Transcriptional regulator ERG antibody (clone rERG/6483) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.