Transcriptional activator GLI3 Gli3 Antibody

• Catalog Number: orb76279
• Category: Antibodies
• Description: Transcriptional activator GLI3 Gli3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Gli3 (40-57aa SNEDESPGQTYHRERRNA), different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 169863 MW
• UniProt ID: P10071
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Transcriptional activator GLI3;GLI3 form of 190 kD
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of rat Testis Tissue Lysate (Lane1), A549 Cell Lysate (Lane2) and SW620 Cell Lysate (Lane3) using GLI3 antibody