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Catalog Number | orb614140 |
---|---|
Category | Antibodies |
Description | TRAF6 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IHC, WB |
Reactivity | Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived mouse TRAF6 recombinant protein (Position: Q14-D512). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | "Western blot, 0.25-0.5μg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat Flow Cytometry, 1-3μg/1x106 cells, Mouse Direct ELISA, 0.1-0.5μg/ml, Mouse" |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 60-65 kDa |
UniProt ID | P70196 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | TNF receptor-associated factor 6; E3 ubiquitin-pro Read more... |
Note | For research use only |
Application notes | "Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users." . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis using anti-TRAF6 antibody.Lane 1:rat RH35 cell, Lane 2:mouse thymus tissue, Lane 3:mouse spleen tissue, Lane 4:mouse RAW246.7 cell.
IHC analysis of TRAF6 using anti-TRAF6 antibody.
IHC analysis of TRAF6 using anti-TRAF6 antibody.
Flow Cytometry analysis of HEPA 1-6 cells using anti-TRAF6 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.
ICC, IHC-Fr, IHC-P, WB | |
Mouse | |
Rabbit | |
Monoclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IF, WB | |
Bovine, Porcine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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