TRAF4 Antibody

• Catalog Number: orb1525900
• Category: Antibodies
• Description: TRAF4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: between 420 and 470
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: TRAF4
• UniProt ID: Q9BUZ4
• NCBI: NP_004286.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: cysteine-rich domain associated with ring and TRAF
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human TRAF4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRAF4 antibody orb1525900 used for IP at 3 µg per reaction.

Detection of human TRAF4 by immunohistochemistry.Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-TRAF4 (Cat. No. orb1525900) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB