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Catalog Number | orb214703 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to TRAF3 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Porcine, Rat, Sheep |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TRAF3. The exact sequence is proprietary. |
Dilution range | WB: 1-500-1-1000, IF/ICC: 1-100-1-500 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | TRAF3 |
Entrez | 22031, 7187 |
UniProt ID | Q13114, Q60803 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | anti CAP1 antibody, anti CRAF1 antibody, anti TNF Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of TRAF3 expression in MCF7 (A), HeLa (B), Raji (C), NIH3T3 (D), mouse lung (E), rat colon (F), PC12 (G) whole cell lysates. (Predicted band size: 55; 64 kD; Observed band size: 70 kD)
Immunofluorescent analysis of TRAF3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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