TPD52L2 Antibody

• Catalog Number: orb1247626
• Category: Antibodies
• Description: TPD52L2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-SGDKPLSDPAP
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 28kDa band observed in Human Breast Cancer and Human Brain lysates (calculated MW of 24.9kDa according to NP_955392.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. See also our D52 and D53 products.Immunohistochemistry:Paraffin embedded Human Spleen, Tonsil and Brain (Cortex). Recommended concentration: 3.75ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TPD52L2
• UniProt ID: O43399
• NCBI: NP_955394.1, NP_955395.1, NP_955393.1, NP_003279.2, NP_955392.1, NP_955391.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: TPD52L2, D54, tumor protein D52-like 2 , DKFZp686A
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 28kDa band observed in Human Breast Cancer and Human Brain lysates (calculated MW of 24.9kDa according to NP_955392.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. See also our D52 and D53 products.Immunohistochemistry:Paraffin embedded Human Spleen, Tonsil and Brain (Cortex). Recommended concentration: 3.75ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Expiration Date: 12 months from date of receipt.

orb1247626 (3.75 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247626 (0.1 ug/ml) staining of Human Breast Cancer lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247626 (3.75 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247626 (3.75 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.