TOX3 Antibody

• Catalog Number: orb1252546
• Category: Antibodies
• Description: TOX3 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: TOX3/1123
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG2b, kappa
• Immunogen: A recombinant fragment (139 amino acid residues around aa 200-400) from the human protein was used as the immunogen for the TOX3 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the TOX3 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 or 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TOX3
• UniProt ID: O15405
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: TOX high mobility group box family member 3, CAG t
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the TOX3 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 or 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human gastric carcinoma stained with TOX3 antibody (TOX3/1123).

Formalin-fixed, paraffin-embedded human gall bladder stained with TOX3 antibody (TOX3/1123).

Formalin-fixed, paraffin-embedded human cervical carcinoma stained with TOX3 antibody (TOX3/1123).

SDS-PAGE Analysis of Purified, BSA-Free TOX3 Antibody (clone TOX3/1123). Confirmation of Integrity and Purity of the Antibody.