Total Iron-Binding Capacity (TIBC) Colorimetric Assay Kit

• Catalog Number: orb1173198
• Category: Assays and Kits
• Description: Total Iron-Binding Capacity (TIBC ) Assay Kit provides a simple, sensitive, rapid TIBC detection method, suitable for detecting serum samples.
• Hazard Information: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
• Storage: The kit is valid for 6 months. Please refer to the table below Materials supplied and Storage conditions to store all the components.
• Note: For research use only
• Application notes: Total Iron-Binding Capacity (TIBC ) Assay Kit provides a simple, sensitive, rapid TIBC detection method, suitable for detecting serum samples. The principle is that under alkaline conditions, serum transferrin can be bonded to Fe3+, and the remaining unbound Fe3+ can be reduced to Fe2+, the bonded Fe3+ could be released after acidification ,and then be reduced into Fe2+ . A purple compound could be produced by the reaction between Fe2+ and philo oxazine ,and this purple compound has a special wave at 562 nm. Respectively reading the OD562nm values A1 and A2 , and A1 is based on the unbound Fe3+ , A2 is based on the bounded Fe3+ . And the concentration of TIBC is proportionate to the difference between A2 and A1.
• Expiration Date: Please enquire.