Topoisomerase 2 alpha (pS1106) antibody

• Catalog Number: orb665510
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Topoisomerase 2 alpha (Phospho-S1106)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S1106 of human Topoisomerase 2 alpha protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1000, IHC-P: 1-50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: TOP2A
• Entrez: 7153, 21973, 360243
• UniProt ID: P41516, Q01320, P11388
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-TOP2 antibody, anti-DNA topoisomerase 2-alpha
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Topoisomerase 2 alpha (Phospho-S1106) expression in Myla2059 (A), HuT78 (B), mouse testis (C), rat testis (D) whole cell lysates. (Predicted band size: 174 kD; Observed band size: 180 kD)

Immunohistochemical analysis of Topoisomerase 2 alpha (Phospho-S1106) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.