TOP2A Antibody

SKU: orb1252175

Description

TOP2A Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Reactivity: Human, Mouse
• Application Notes:
  Flow Cytometry: 0.5-1ug/10^6 cells in 0.1mlIF: 1-2 µg/mLWestern blot: 1-2 µg/mLIHC (FFPE): 0.5-1 µg/mLThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Topoisomerase II alpha antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone No.: TOP2A/1361
• Immunogen: Amino acids 1352-1493 from the human protein were used as the immunogen for this Topoisomerase II alpha antibody.
• Target: TOP2A
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme, TOP2A, TOP2

Western blot testing of 1) human HepG2, 2) HeLa and 3) mouse NIH3T3 cell lysate with Topoisomerase II alpha antibody (clone TOP2A/1361). Expected molecular weight ~174 kDa.

IHC testing of FFPE human tonsil with Topoisomerase II alpha antibody (clone TOP2A/1361). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human bladder carcinoma with Topoisomerase II alpha antibody (clone TOP2A/1361). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Quick Database Links

Gene Symbol

TOP2A

UniProt

P11388