TOP1 Antibody

• Catalog Number: orb1526355
• Category: Antibodies
• Description: TOP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: between 100 and 150
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: TOP1
• UniProt ID: P11387
• NCBI: NP_003277.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: topoisomerase (DNA) I; type I DNA topoisomerase; D
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human TOP1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-TOP1 antibody orb1526355 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. TOP1 was less efficiently immunoprecipitated by rabbit anti-TOP1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).