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Catalog Number | orb1526354 |
---|---|
Category | Antibodies |
Description | TOP1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human, Mouse |
Immunogen | between 715 and 765 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | TOP1 |
UniProt ID | P11387 |
NCBI | NP_003277.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | topoisomerase (DNA) I; type I DNA topoisomerase; D Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of mouse TOP1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOP1 antibody orb1526354 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human TOP1 by western blot. Samples: Whole cell lysate (50, 15, 5 µg) from HeLa and (50 µg) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOP1 antibody orb1526354 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human TOP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TOP1 antibody orb1526354 used for IP at 6 µg per reaction.
ICC, IHC-Fr, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse | |
Rabbit | |
Monoclonal | |
Unconjugated |
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