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Catalog Number | orb2290705 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a full length recombinant TOE1. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 1D8 |
Tested applications | ELISA, IHC-P, WB |
Reactivity | Human |
Isotype | IgG1 kappa |
Immunogen | TOE1 (AAH09364, 1 a.a. ~ 510 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | MAADSDDGAVSAPAASDGGVSKSTTSGEELVVQVPVVDVQSNNFKEMWPSLLLAIKTANFVAVDTELSGLGDRKSLLNQCIEERYKAVCHAARTRSILSLGLACFKRQPDKGEHSYLAQVFNLTLLCMEEYVIEPKSVQFLIQHGFNFNQQYAQGIPYHKGNDKGDESQSQSVRTLFLELIRARRPLVLHNGLIDLVFLYQNFYAHLPESLGTFTADLCEMFPAGIYDTKYAAEFHARFVASYLEYAFRKCERENGKQRAAGSPHLTLEFCNYPSSMRDHIDYRCCLPPATHRPHPTSICDNFSAYGWCPLGPQCPQSHDIDLIIDTDEAAAEDKRRRRRRREKRKRALLNLPGTQTSGEAKDGPPKKQVCGDSIKPEETEQEVAADETRNLPHSKQGNKNDLEMGIKAARPEIADRATSEVPGSQASPNPVPGDGLHRAGFDAFMTGYVMAYVEVSQGPQPCSSGPWLPECHNKVYLSGKAVPLTVAKSQFSRSSKAHNQKMKLTWGSS |
NCBI | AAH09364 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunoperoxidase of monoclonal antibody to TOE1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.3 ug/ml]
TOE1 monoclonal antibody (M01), clone 1D8 Western Blot analysis of TOE1 expression in Jurkat.
Western Blot analysis of TOE1 expression in transfected 293T cell line by TOE1 monoclonal antibody (M01), clone 1D8. Lane 1: TOE1 transfected lysate(56.5 KDa). Lane 2: Non-transfected lysate.
Western blot analysis of TOE1 over-expressed 293 cell line, cotransfected with TOE1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TOE1 monoclonal antibody (M01), clone 1D8 (Cat # orb2290705). GAPDH (36.1 kDa) used as specificity and loading control.
Western Blot detection against Immunogen (81.84 KDa).