TNNT1 antibody

• Catalog Number: orb79336
• Category: Antibodies
• Description: Goat polyclonal antibody to TNNT1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Canine, Human, Porcine
• Dilution range: ELISA: 1:128000, WB: 0.03-0.1 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 32.9; 31.2; 30.1
• Target: TNNT1
• Entrez: 7138
• Protein Sequence: EKAQELSDWIHQLE
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti TNNT1 antibody, anti troponin T type 1 (skele
• Note: For research use only
• Application notes: WB: Preliminary experiments gave an approx 50kDa band in Human Skeletal Muscle lysates after 0.1μg/ml antibody staining.Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.9kDa according to NP_003274.3.The 50kDa band was successfully blocked by incubation with the immunizing peptide.We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates.Have any further splice variants/modified forms been reported.Experiment Notes: This antibody is expected to recognize all reported isoforms (NP_003274.3,NP_001119604.1,NP_001119605.1).
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Human Skeletal Muscle lysate using TNNT1 antibody

Immunohistochemical staining of Human Skeletal Muscle of TNNT1 antibody.