TNNI3 Antibody

• Catalog Number: orb1672006
• Category: Antibodies
• Description: TNNI3 Antibody
• Clonality: Recombinant
• Clone Number: scFv 180
• Tested applications: WB
• Reactivity: Human
• Isotype: IgG1 lambda
• Immunogen: Cardiac Troponin I peptide (KISASRKLQLKT).
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: TNNI3
• UniProt ID: P19429
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TNNI3, cTnI, cTn-I
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot using anti-Cardiac Troponin I antibody scFv 180. Human heart lysate (35 ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of scFv 180 (orb1672006) at 0.001 ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for Cardiac Troponin I is 24.0 kDa. orb1672006 successfully detected Cardiac Troponin I in human heart lysate.

Immunofluorescence staining of fixed HeLa cells with anti-Cardiac Troponin I antibody scFv 180. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton and stained with the chimeric mouse IgG1 version of scFv 180 (orb1672006) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672006- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody () followed by Alexa Fluor® 488 secondary antibody.