TNFSF13B Antibody

• Catalog Number: orb1272595
• Category: Antibodies
• Description: TNFSF13B Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hBAFF (human BAFF).
• Concentration: batch dependent
• Dilution range: ELISA:To detect hBAFF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hBAFF. Western Blot:To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: TNFSF13B
• UniProt ID: Q9Y275
• NCBI: Q9Y275
• Storage: BAFF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: DTL, BAFF, BLYS, CD257, TALL1, THANK, ZTNF4, TALL-
• Note: For research use only
• Application notes: ELISA:To detect hBAFF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hBAFF. Western Blot:To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect hBAFF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human BAFF (orb1272594) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBAFF.

To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.