TNFSF10 / TRAIL Antibody

• Catalog Number: orb1538975
• Category: Antibodies
• Description: TNFSF10 / TRAIL Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: E.coli derived recombinant Human TRAIL/Apo2L.
• Dilution range: ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml)
• Conjugation: Unconjugated
• Target: TNFSF10 / TRAIL
• Storage: Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from PBS, pH 7.2. Lyophilized from PBS, pH 7.2
• Alternative names: TNFSF10, Apo-2L, APO2L, Apo-2 ligand, CD253, CD253
• Note: For research use only
• Application notes: Resuspension: Reconstitute with sterile water. Further information: Immunohistochemistry: LS-B7420 in an antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human sTRAIL/Apo2L (10 ng/ml), a concentration of 150 ng/ml of this antibody is required. ELISA: To detect hsTRAIL/Apo2L by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sTRAIL/Apo2L (LS-C104497) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsTRAIL/Apo2L. Western Blot: To detect hsTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTRAIL/Apo2L is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.