TNFSF10 Antibody

• Catalog Number: orb1238614
• Category: Antibodies
• Description: TNFSF10 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: IgG
• Immunogen: TRAIL antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human TRAIL.The immunogen is located within the last 50 amino acids of Trail.
• Concentration: 1 mg/ml
• Dilution range: TRAIL antibody can be used for detection of TRAIL by Western blot at 1 - 5 μg/mL dilution. Antibody can also be used for immunohistochemistry starting at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 32 kDa Observed: 35 kDa
• Target: TNFSF10
• UniProt ID: P50591
• NCBI: NP_003801
• Storage: Trail antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Trail Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Trail Antibody: TL2, APO2L, CD253, TRAIL, Apo-2L,
• Note: For research use only
• Application notes: TRAIL antibody can be used for detection of TRAIL by Western blot at 1 - 5 μg/mL dilution. Antibody can also be used for immunohistochemistry starting at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of TRAIL in HeLa cell lysate containing 10, 2.5, or 1 ng of recombinant protein containing extracellular domain of TRAIL with TRAIL antibody at 1 µg/mL.

Immunohistochemistry of TRAIL in human brain tissue with TRAIL antibody at 20 µg/mL.

Western blot analysis of TRAIL in human brain tissue lysate with TRAIL antibody at (A) 2.5 and (B) 5 µg/mL.

Immunofluorescence of TRAIL in human brain tissue with Trail antibody at 20 µg/mL. Red: Trail Antibody (orb1238614) Blue: DAPI staining.