TNFRSF9 Antibody

• Catalog Number: orb1671559
• Category: Antibodies
• Description: TNFRSF9 Antibody
• Clonality: Recombinant
• Clone Number: 4B4-1-1
• Tested applications: Blocking, ELISA, FC, IHC
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: The original antibody was generated by immunizing BALB/c mice with a fusion protein of human 4-1BB antigen and glutathione s transferase (GST).
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: TNFRSF9
• UniProt ID: Q07011
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD137, MGC2172, Tumor necrosis factor receptor sup
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed Jurkat cells with anti-4-1BB antibody 4B4-1-1 (Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric r version of 4B4-1-1 (orb1671559) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671559- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-4-1BB antibody 4B4-1-1 (Human thymus(A)- lymph node(B) and tonsil(C) tissue lysate (35 ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of 4B4-1-1 (orb1671559) at 0.3 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.