TNFRSF7 monoclonal antibody (M01), clone 1E2-A3

• Catalog Number: orb2294892
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant TNFRSF7.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Immunogen: TNFRSF7 (AAH12160, 1 a.a. ~ 260 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MARPHPWWLCVLGTLVGLSATPAPKSCPERHYWAQGKLCCQMCEPGTFLVKDCDQHRKAAQCDPCIPGVSFSPDHHTRPHCESCRHCNSGLLVRNCTITANAECACRNGWQCRDKECTECDPLPNPSLTARSSQALSPHPQPTHLPYVSEMLEARTAGHMQTLADFRQLPARTLSTHWPPQRSLCSSDFIRILVIFSGMFLVFTLAGALFLHQRRKYRSNKGESPVEPAEPCRYSCPREEEGSTIPIQEDYRKPEPACSP
• Tested applications: ELISA, WB
• Clone Number: 1E2-A3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• NCBI: AAH12160

Detection limit for recombinant GST tagged CD27 is 0.3 ng/ml as a capture antibody.

TNFRSF7 monoclonal antibody (M01), clone 1E2-A3 Western Blot analysis of TNFRSF7 expression in Jurkat.

Western Blot analysis of TNFRSF7 expression in transfected 293T cell line by TNFRSF7 monoclonal antibody (M01), clone 1E2-A3. Lane 1: TNFRSF7 transfected lysate(29.2 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of TNFRSF7 over-expressed 293 cell line, cotransfected with TNFRSF7 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TNFRSF7 monoclonal antibody (M01), clone 1E2-A3 (Cat # orb2294892). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (54.34 KDa).