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    TNF Receptor II/TNFRSF1B Antibody

    Catalog Number: orb865475

    DispatchUsually dispatched within 5-10 working days
    $ 520.00
    Catalog Numberorb865475
    CategoryAntibodies
    DescriptionTNF Receptor II/TNFRSF1B Antibody
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsELISA, FC, WB
    ReactivityHuman, Mouse, Rat
    IsotypeRabbit IgG
    ImmunogenE.coli-derived human TNF Receptor II/TNFRSF1B recombinant protein (Position: L23-A189).
    ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
    Dilution rangeWestern blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Flow Cytometry, 1-3 μg/1x106 cells, Human Direct ELISA, 0.1-0.5 μg/ml, Human
    Form/AppearanceLyophilized
    ConjugationUnconjugated
    MW75 kDa
    UniProt IDP20333
    StorageAt -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
    NoteFor research use only
    Application notesTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
    Expiration Date12 months from date of receipt.
    TNF Receptor II/TNFRSF1B Antibody

    Western blot analysis of TNF Receptor II/TNFRSF1B using anti-TNF Receptor II/TNFRSF1B antibody (orb865475). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor II/TNFRSF1B antigen affinity purified polyclonal antibody (Catalog # orb865475) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for TNF Receptor II/TNFRSF1B at approximately 75 kDa. The expected band size for TNF Receptor II/TNFRSF1B is at 75 kDa.

    TNF Receptor II/TNFRSF1B Antibody

    Flow Cytometry analysis of K562 cells using anti-TNF Receptor II/TNFRSF1B antibody (orb865475). Overlay histogram showing K562 cells stained with orb865475 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TNF Receptor II/TNFRSF1B Antibody (orb865475, 1 μg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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      WB

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      Polyclonal

      Unconjugated

      50 μl, 100 μl, 200 μl
    • TNF Receptor II/TNFRSF1B Antibody [orb308810]

      IHC,  WB

      Mouse, Rat

      Rabbit

      Polyclonal

      Unconjugated

      10 μg, 100 μg
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      ICC,  IF,  WB

      Human

      Polyclonal

      Unconjugated

      200 μl, 100 μl, 50 μl
    • TNF Receptor II/TNFRSF1B Antibody [orb18148]

      IHC,  WB

      Canine

      Human

      Rabbit

      Polyclonal

      Unconjugated

      10 μg, 100 μg
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      FC,  ICC,  IF,  IHC,  IP,  WB

      Human, Mouse, Rat

      Rabbit

      Monoclonal

      Unconjugated

      30 μl, 100 μl
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