TNF p55 Receptor antibody

• Catalog Number: orb420295
• Category: Antibodies
• Description: TNF p55 Receptor antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human sTNFRp55 expressed in Chinese Hamster Cells.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Human TNF p55 Receptor Antibody is an IgG preparation of whole rabbit serum purified by Protein G chromatography. This antibody is directed against the extracellular domain of the cell bound human TNFRp55. In general, this antibody also detects primate sTNFRp55. The antibody does not recognize human sTNFRp75. In ELISA formats and other immunoreactive assays, this antibody will detect the extracellular domain of the TNFRp55 found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid. This antibody can be used for FACS analysis. Caution should be exhibited as the F( c) domain of the rabbit IgG molecule may interact with cells non-specifically.
• Conjugation: Unconjugated
• UniProt ID: P19438
• NCBI: P19438.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-TNFp55 Receptor Antibody, TBP1 antibod
• Note: For research use only
• Application notes: Anti-TNF p55 Receptor (sTNFRp55) antibody has been tested for use in ELISA, radioimmunoassays, immunoblotting, flow cytometry and immunoprecipitation. Reactivity in other immunoassays is unknown. This product has been assayed for the ability to immunoprecipitate antigen and a dilution range of 1:400 to 1:800 is suggested. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background. This product has been assayed by immunoblot and a working dilution range of 1:200 to 1:400 is suggested for this application. This product has been assayed by ELISA against soluble (extracellular) sTNFRp55 at a dilution range of 1:2,000 to 1:8,000. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This product has been assayed by radioimmunoassay against antigen. A dilution of 1:8,000 is suggested for this immunoassay.
• Expiration Date: 12 months from date of receipt.