Tnf Antibody (Biotin)

• Catalog Number: orb1272286
• Category: Antibodies
• Description: Tnf Antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Mouse
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mTNF-α (murine TNF-alpha).
• Concentration: batch dependent
• Dilution range: ELISA:Sandwich:To detect mTNF-α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine TNF-α (XP-5284) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mTNF-α.Western Blot:To detect mTNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mTNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Biotin
• Target: Tnf
• UniProt ID: P06804
• NCBI: P06804
• Storage: TNF-a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: DIF, Tnfa, TNF-a, TNFSF2, Tnfsf1a, TNFalpha, TNF-a
• Note: For research use only
• Application notes: ELISA:Sandwich:To detect mTNF-α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine TNF-α (XP-5284) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mTNF-α.Western Blot:To detect mTNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mTNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect Murine TNF-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Murine TNF-alpha (orb1272287) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Murine TNF-alpha.

To detect Murine TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Murine TNF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Murine TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Murine TNF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.