TNF-alpha Antibody

SKU: orb2645159

Description

Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IF, IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Immunofluorescence: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  The optimal dilution of the anti-TNF-alpha antibody for each application should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgM, kappa
• Clone No.: SPM543
• Immunogen: Amino acids 115-130 of the human protein was used as the immunogen for this anti-TNF alpha antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Immunofluorescent staining of FFPE human HepG2 cells with anti-TNF-alpha antibody (green) and RedDot nuclear stain (red).

IHC staining of FFPE human histiocytoma with anti-TNF-alpha antibody. HIER: boil tissue sections in pH9 EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P01375