TNAP antibody (APC)

• Catalog Number: orb699836
• Category: Antibodies
• Description: Mouse monoclonalantibody against TNAP conjugated to APC
• Clonality: Monoclonal
• Clone Number: W8B2B10
• Tested applications: FC
• Reactivity: Canine, Goat, Human, Monkey, Porcine, Sheep
• Isotype: Mouse IgG1
• Immunogen: WERI-RB-1 retinoblastoma cell line
• Concentration: 0.1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 2-5 μg/ml.
• Purity: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: APC
• Target: TNAP
• Entrez: 249
• UniProt ID: P05186
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Tissue Non-specific Alkaline Phosphatase anti
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 2-5 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of TNAP positive HeLa cells (red-filled) from TNAP negative HeLa cells (black-dashed) in flow cytometry analysis (surface staining) of HeLa cellular suspension stained using anti-TNAP (W8B2B10) APC antibody (concentration in sample 0.56 μg/ml).

Flow cytometry surface staining patterns of HeLa cells stained using anti-TNAP (W8B2B10) APC antibody (concentration in sample 0.56 μg/ml, left) or mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 0.56 μg/ml, same as TNAP APC concentration, right).