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Catalog Number | orb1246913 |
---|---|
Category | Antibodies |
Description | TMPRSS5 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Canine |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-LDWIHDTAQDSLL |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Experiments gave bands at approx 50kDa and 29kDa in Human Brain (Cerebral Cortex). These bands correspond to earlier findings in literature with different antibodies (Yamaguchi et al, J Biol Chem. 2002 Mar 1;277(9):6806-12; PMID: 11741986). This protein has a calculated MW of 49.6kDa according to NP_110397.1. Recommended concentration: 0.5-1.5ug/ml.Immunohistochemistry:Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 5ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | TMPRSS5 |
UniProt ID | Q9H3S3 |
NCBI | NP_110397.2 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | TMPRSS5, SPINESIN, transmembrane protease, serine Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Experiments gave bands at approx 50kDa and 29kDa in Human Brain (Cerebral Cortex). These bands correspond to earlier findings in literature with different antibodies (Yamaguchi et al, J Biol Chem. 2002 Mar 1;277(9):6806-12; PMID: 11741986). This protein has a calculated MW of 49.6kDa according to NP_110397.1. Recommended concentration: 0.5-1.5ug/ml.Immunohistochemistry:Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 5ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1246913 staining (0.5 ug/ml) of Human Brain (Cerebral Cortex) lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
orb1246913 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb1246913 (5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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