TMEM176A Rabbit Polyclonal Antibody

• Catalog Number: orb158621
• Category: Antibodies
• Description: TMEM176A Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from MOUSE TMEM176A (1-100/235aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 26 kDa
• Target: TMEM176A
• UniProt ID: Q9DCS1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: GS188; HCA112; Hepatocellular carcinoma-associated
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TMEM176A) Polyclonal Antibody, Unconjugated (orb158621) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Protein: HepG2 (human) lysates at 30 ug, Primary: rabbit Anti-TMEM176A (orb158621) at 1:300, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000, Predicted band size: 26 kD, Observed band size: 26 kD.

Protein: mouse kidney lysates, 30 ug, Primary: Anti-TMEM176A (orb158621) at 1:300, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:5000, ECL excitated the fluorescence, Predicted band size: 24kD, Observed band size: 24kD.

Sample: U937 (Human) Cell Lysate at 30 ug, Primary: Anti-TMEM176A (orb158621) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 26 kD, Observed band size: 25 kD.

Tissue/Cell: mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-TMEM176A Polyclonal Antibody, Unconjugated (orb158621) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.