Tmb Prestained Red Elisa Peroxidase Substrate

SKU: orb348655

Description

Tmb Prestained Red Elisa Peroxidase Substrate

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  TMB Prestained Red ELISA Peroxidase Substrate produces a soluble blue end product of ELISA, read at 370nm or 655nm.

Key Properties

• Concentration: 1X
• Conjugation: Unconjugated
• Purity: pH: 4.0 +/- 0.2 Stability @ 18° to 26°C: PASS Stability @ 4°C: PASS QC Raw Material: PASS Absorbance check of final product: PASS Performance Data per ELISA: PASS

Storage & Handling

• Storage: Store container at 4° C prior to opening. Protect from moisture and light. No special shipping conditions or precautions are required.
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

3,3’,5,5’-Tetramethylbenzidine; TMB-E; TMBE; RTMBE; R-TMB-E

Biorbyt produces a wide variety of buffers and substrates for use in ELISAs. Antigen was diluted in ELISA Microwell Coating Stabilizer (p/n orb348581) added to the microwell plate and incubated overnight at 4°C. The plate was then blocked with ELISA Microwell Blocking Buffer with Stabilizer (p/n orb348584) for 2 hours. The primary antibody was diluted in PBS Fish Gel Concentrate (1:10)(p/n orb348587), added to the plate, and allowed to incubate 1 hour at room temperature. HRP conjugated secondary antibody was diluted in HRP Conjugate Stabilizer, added to the plate, and allowed to incubate for 30 minutes at room temperature. TMB Prestained Red ELISA Peroxidase Substrate (p/n orb348654) (Rows A-D) and TMB ELISA Peroxidase Substrate (p/n orb348651) (rows E-H) were added to the plate and allowed to incubate for 30 minutes at room temperature. The reaction was then stopped with 1M HCl and read at 450nm. The Red TMBE has comparable background to Biorbyt's regular TMBE.