Tmb Prestained Red Elisa Peroxidase Substrate

• Catalog Number: orb348655
• Category: Assays and Kits
• Description: Tmb Prestained Red Elisa Peroxidase Substrate
• Tested applications: ELISA
• Concentration: 1x
• Dilution range: ELISA: 1x
• Form/Appearance: Liquid - light red colored
• Purity: pH: 4.0 +/- 0.2 Stability @ 18° to 26°C: PASS Stability @ 4°C: PASS QC Raw Material: PASS Absorbance check of final product: PASS Performance Data per ELISA: PASS
• Conjugation: Unconjugated
• Hazard Information: Non-Toxic
• Storage: Store container at 4° C prior to opening. Protect from moisture and light. No special shipping conditions or precautions are required.
• Buffer/Preservatives: Proprietary buffer with enhancer
• Alternative names: 3,3’,5,5’-Tetramethylbenzidine; TMB-E; TMBE; RTMBE
• Note: For research use only
• Application notes: TMB Prestained Red ELISA Peroxidase Substrate produces a soluble blue end product of ELISA, read at 370nm or 655nm.
• Expiration Date: Please enquire.

Biorbyt produces a wide variety of buffers and substrates for use in ELISAs. Antigen was diluted in ELISA Microwell Coating Stabilizer (p/n orb348581) added to the microwell plate and incubated overnight at 4°C. The plate was then blocked with ELISA Microwell Blocking Buffer with Stabilizer (p/n orb348584) for 2 hours. The primary antibody was diluted in PBS Fish Gel Concentrate (1:10)(p/n orb348587), added to the plate, and allowed to incubate 1 hour at room temperature. HRP conjugated secondary antibody was diluted in HRP Conjugate Stabilizer, added to the plate, and allowed to incubate for 30 minutes at room temperature. TMB Prestained Red ELISA Peroxidase Substrate (p/n orb348654) (Rows A-D) and TMB ELISA Peroxidase Substrate (p/n orb348651) (rows E-H) were added to the plate and allowed to incubate for 30 minutes at room temperature. The reaction was then stopped with 1M HCl and read at 450nm. The Red TMBE has comparable background to Biorbyt's regular TMBE.