Tmb Membrane Peroxidase Substrate

SKU: orb348656

Description

Tmb Membrane Peroxidase Substrate

Key Properties

• Conjugation: Unconjugated

Storage & Handling

• Storage: Store container at 4° C prior to opening. Protect from moisture and light. No special shipping conditions or precautions are required.
• Form/Appearance: Liquid - clear
• Buffer/Preservatives: Proprietary
• Concentration: 1X
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

3,3',5,5'-Tetramethylbenzidine, TMB-M, TMBM

Immunological Features in the H1N1 Influenza-Infected SOTR Cohort (A and B) Pre-existing antibody titers in SOTRs with H1N1pdm09 influenza virus infection (n = 40) compared with non-infected matched SOTRs controls (n = 9). The antibody level for each patient, GMT, and 95% CI are shown (A) for the indicated ELISA assay on the left y axis (full-length HA, stalk HA, and NA recombinant proteins as antigen) and HA-inhibiting (HAI) antibody titers on the right y axis. The HAI assay was performed using the whole H1N1pdm09 reference strain virus. (B) shows antibody-dependent cellular cytotoxicity (ADCC), measured by a reporter assay for full-length HA, stalk HA, and NA. (C–F) Pre-existing antibody responses in SOTRs with LRSs at baseline. The antibody level for each patient and time point, GMT, and 95% CI are shown for (C) full-length HA ELISA, (D) stalk HA ELISA, (E) HAI, and (F) NA ELISA (ns, not significant; ∗∗∗p = 0.01; ∗∗p = 0.006; ∗p = 0.03). Experiments were performed in triplicates. 3, 3', 5, 5'-Tetramethylbenzidine (p/n orb348656).

Biorbyt TMB membrane substrate, chemiluminescent substrate and secondary antibodies detect rabbit primary antibodies in a variety of platforms. Shown here is a serial 1:1 dilution of control rabbit IgG protein (orb2652747, 250 ng starting total load) co incubated with Biorbyt HRP conjugated Goat anti Rabbit IgG (orb347654) and Dylight 649 conjugated goat anti Rabbit 1:20K in orb348637.