Tmb Elisa Peroxidase Substrate

SKU: orb348653

Description

Tmb Elisa Peroxidase Substrate

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  TMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay conditions. No dilutions are required, TMB ELISA Peroxidase Substrate comes ready to use.

Key Properties

• Concentration: 1X
• Conjugation: Unconjugated
• Purity: pH: 4.0 +/- 0.05 Stability @ 18° to 26°C: PASS Stability @ 4°C: PASS QC Raw Material: PASS Absorbance check of final product: PASS Performance Data per ELISA: PASS

Storage & Handling

• Storage: Store container at 4° C prior to opening. Protect from moisture and light. No special shipping conditions or precautions are required.
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

TMBE Peroxidase Substrate, TMB ELISA Peroxidase Substrate

ELISA results of Mouse Anti-SARS CoV-2 Spike Protein (S2) Antibody using orb348651. Each well was coated in duplicate with 0.5 µg of rec SARS CoV-2 Whole Spike protein [RED line], rec SARS CoV-2 Spike (S1) [GREEN line], rec SARS CoV-2 Spike (S1) RBD protein [BLUE line], and rec SARS CoV-2 Spike (S2) glycoprotein [PURPLE line]. The working dilution for SARS-CoV-2 whole spike is 1:190000 and SARS CoV-2 Spike (S2) glycoprotein is 1:120000. The starting dilution of antibody was 0.55 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Rabbit Anti-Mouse IgG HRP conjugated (p/n orb347506) at 1:8000 and TMB substrate (p/n orb348651). SARS CoV-2 Spike (S2) EC50: 8 ng/mL; SARS CoV-2 Whole Spike EC50: 5 ng/mL.