Tmb Elisa Peroxidase Substrate

• Catalog Number: orb348650
• Category: Assays and Kits
• Description: Tmb Elisa Peroxidase Substrate
• Conjugation: Unconjugated
• Form/Appearance: Liquid - clear to very light blue colored liquid
• Concentration: 1x
• Buffer/Preservatives: Proprietary buffer with enhancer
• Purity: pH: 4.0 +/- 0.2 Stability @ 18° to 26°C: PASS Stability @ 4°C: PASS QC Raw Material: PASS Absorbance check of final product: PASS Performance Data per ELISA: PASS
• Tested applications: ELISA
• Dilution range: ELISA: 1X
• Application notes: TMB ELISA Peroxidase Substrate comes ready to use. No dilutions are required. TMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay conditions.
• Storage: Store container at 4° C prior to opening. Protect from moisture and light. No special shipping conditions or precautions are required.
• Alternative names: 3,3’,5,5’-Tetramethylbenzidine, TMB ELISA substrat
• Hazard Information: Non-Toxic
• Note: For research use only

ELISA results of Mouse Anti-SARS CoV-2 Spike Protein (S2) Antibody using orb348651. Each well was coated in duplicate with 0.5 µg of rec SARS CoV-2 Whole Spike protein [RED line], rec SARS CoV-2 Spike (S1) [GREEN line], rec SARS CoV-2 Spike (S1) RBD protein [BLUE line], and rec SARS CoV-2 Spike (S2) glycoprotein [PURPLE line]. The working dilution for SARS-CoV-2 whole spike is 1:190000 and SARS CoV-2 Spike (S2) glycoprotein is 1:120000. The starting dilution of antibody was 0.55 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Rabbit Anti-Mouse IgG HRP conjugated (p/n orb347506) at 1:8000 and TMB substrate (p/n orb348651). SARS CoV-2 Spike (S2) EC50: 8 ng/mL; SARS CoV-2 Whole Spike EC50: 5 ng/mL.