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Catalog Number | orb1108976 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody against TLR9 conjugated to FITC |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | FC, IF, IHC-Fr, PS, WB |
Reactivity | Human |
Isotype | Mouse IgG2a |
Form/Appearance | 1.0 ml (100 µg/ml) 0.2 µm filtered FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide. |
Conjugation | FITC |
Storage | Product should be stored at 4degC. Under recommended storage conditions, product is stable for one year. |
Note | For research use only |
Application notes | F: 10 um sections were fixed with acetone for 10 minutes. PBS washed sections were incubated with 5G5 1:100 in 1% BSA for 30 minutes at RT. (Ref.7) IF: cells were fixed with 2% formalin for 15 minutes at RT and permeabilized with a mAb (4ug/400ul) containing buffer (PBS, 0.2% BSA, 0.2% saponin)- for 1 hour. (Ref.1) FC: RAW264.7 cells were fixed for 15 minutes with 4% formalin and permeabilized (PBS, 0.5%BSA, 0,5% saponin) at RT. (Ref.1) P: paraffin embedded tissues 5um;m sections were made. After antigen retrieval (0.01mol/l, pH6 sodium citrate) and quenching of endogenous peroxidase, sections were blocked with 0.5% ovalbumin and 0.1% gelatin for 20 minutes at RT. Sections were incubated with 5G5 for 1 hour at 37degC. (Ref.3) W: reduced lysates were resolved by 10% SDS-PAGE and blotted on nitrocellulose. After blocking with 5% skimmed milk TLR9 was detected with 2 µg/ml 5G5. (Ref.1) |
Expiration Date | 12 months from date of receipt. |
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