TLR9 antibody (Biotin)

• Catalog Number: orb1108977
• Category: Antibodies
• Description: Mouse monoclonal antibody against TLR9 conjugated to Biotin
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: FC, IA, IF, IHC-Fr, PS, WB
• Reactivity: Human
• Isotype: Mouse IgG2a
• Form/Appearance: 0.5 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
• Conjugation: Biotin
• Storage: Product should be stored at 4degC. Under recommended storage conditions, product is stable for one year.
• Note: For research use only
• Application notes: F: 10 um sections were fixed with acetone for 10 minutes. PBS washed sections were incubated with 5G5 1:100 in 1% BSA for 30 minutes at RT. (Ref.7); IF: cells were fixed with 2% formalin for 15 minutes at RT and permeabilized with a mAb (4ug/400ul) containing buffer (PBS, 0.2% BSA, 0.2% saponin)- for 1 hour. (Ref.1); FC: RAW264.7 cells were fixed for 15 minutes with 4% formalin and permeabilized (PBS, 0.5%BSA, 0,5% saponin) at RT. (Ref.1); P: paraffin embedded tissues 5um;m sections were made. After antigen retrieval (0.01mol/l, pH6 sodium citrate) and quenching of endogenous peroxidase, sections were blocked with 0.5% ovalbumin and 0.1% gelatin for 20 minutes at RT. Sections were incubated with 5G5 for 1 hour at 37degC. (Ref.3); W: reduced lysates were resolved by 10% SDS-PAGE and blotted on nitrocellulose. After blocking with 5% skimmed milk TLR9 was detected with 2 µg/ml 5G5. (Ref.1)
• Expiration Date: 12 months from date of receipt.