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Catalog Number | orb1537773 |
---|---|
Category | Antibodies |
Description | TLR9 Antibody (aa268-284, clone 26C593.2) |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 26C593.2 |
Tested applications | FC, IHC, IHC-Fr, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG1,k |
Immunogen | This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-284 (CPRHFPQLHPDTFSHLS) of human TLR9 isoform A (Genbank accession no. AAF78037). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%); Chimpanzee, Marmoset (94%); Sheep, Goat, Zebu, Bovine, Horse (82%). |
Concentration | 1 mg/ml |
Dilution range | Flo, IHC, IHC-Fr (10 - 20 µg/ml), IHC-P (5 µg/ml), WB (1 - 3 µg/ml) |
Conjugation | Unconjugated |
Target | TLR9 |
Storage | Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. |
Buffer/Preservatives | PBS, 0.05% Sodium Azide. PBS, 0.05% Sodium Azide |
Alternative names | TLR9, CD289, CD289 antigen, Scri2a, Toll-like rece Read more... |
Note | For research use only |
Application notes | Further information: Immunohistochemistry: LS-B2341 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2341 was determined to be 5 ug/ml. |
Expiration Date | 12 months from date of receipt. |
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