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Catalog Number | orb1252459 |
---|---|
Category | Antibodies |
Description | TLR2 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | TLR2/221 |
Tested applications | FA, FC, IF, IHC-P, WB |
Reactivity | Canine, Human, Monkey |
Isotype | IgG2a, kappa |
Immunogen | Recombinant human protein was used as the immunogen for the TLR2 antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Functional Activity (Order SAF formulation)Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Optimal dilution of the TLR2 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | TLR2 |
UniProt ID | O60603 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | TLR2 Antibody: TIL4, CD282, TIL4, Toll-like recept Read more... |
Note | For research use only |
Application notes | Functional Activity (Order SAF formulation)Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Optimal dilution of the TLR2 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
Expiration Date | 12 months from date of receipt. |
SDS-PAGE Analysis of Purified, BSA-Free TLR2 Antibody (clone TLR2/221). Confirmation of Integrity and Purity of the Antibody.
ICC, IF, IHC-P, WB | |
Guinea pig, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IHC-Fr, IHC-P, WB | |
Mouse | |
Rabbit | |
Monoclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
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