TLR2 antibody (Biotin)

• Catalog Number: orb1108999
• Category: Antibodies
• Description: Mouse monoclonal antibody against TLR2 conjugated to Biotin
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: FC, Functional Studies, IF, IHC-Fr, IP, PS, WB
• Reactivity: Human
• Isotype: Mouse IgG2a
• Form/Appearance: 0.5 ml (100 µg/ml) 0.2 um filtered protein G purified biotinylated antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
• Conjugation: Biotin
• Storage: Product should be stored at 4degC. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label
• Note: For research use only
• Application notes: FC: 2 ug antibody per 300000 cells; untreated as well as 0.4 % formaldehyde fixed cells can be used; do not permeabilize the cells or use 4 % faraformaldehyde as fixative! (Ref 5); IF: HMECs were fixed with 4% paraformaldehyde in PBS, permeabilized and blocked before staining; antibody concentration used 5-10µg/ml; W: peripheral blood mononuclear cells; non-reduced; 1 µg/ml antibody; size ~90 kDa; F: fixed in acetone; 3 % H2O2 for blockade of endogenous peroxidases; human tonsils as positive control (useful, but clone TL2.3 (HM2066) is preferred; Ref 2); P: HOPE-fixed;alveolar epithelial cells type II in human lung as positive control; 1 µg/ml antibody for 16 hr at 4 degC (Ref 4); IP: lysed monocytes were immunoprecipitated with antibody-conjugated Sepharose; size ~90 kDa (Ref 2); FS: 1 ug/5x107 cells; antibody blocks TNFa release (induced by bacteria) from peripheral blood mononuclear cells (Ref 1)
• Expiration Date: 12 months from date of receipt.