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Catalog Number | orb1108980 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody against TLR1 conjugated to Biotin |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | FC, Functional Studies, IA, IF, IHC-Fr, PS |
Reactivity | Human |
Isotype | Mouse IgG1 |
Form/Appearance | 0.5 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide. |
Conjugation | Biotin |
Storage | Product should be stored at 4degC. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label. |
Note | For research use only |
Application notes | FC: Antibody GD2.F4 stains the extracellular domain of TLR1. Monocytes were resuspended in PBS, 0.1% BSA, 0.02% NAN3 containing 15 µg/ml GD2.F4. As negative control an IgG1 isotype control was used (Ref.1). IHC-P: Tissue sections were pretreated with Target Retrieval solution, 1% Triton X-100 to improve membrane permeability and 0.03% hydrogen peroxide to quench endogenous peroxidases . Tissue sections were blocked with 2% FCS (Ref.6). IHC-F:- Tissue sections were blocked with normal horse serum prior to staining (Ref.4). FS: Antibody GD2.F4e was used to inhibit cytokine production of stimulated PBMCs (Ref.3). |
Expiration Date | 12 months from date of receipt. |
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