TIMP4 Antibody

• Catalog Number: orb19302
• Category: Antibodies
• Description: TIMP4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human TIMP4 recombinant protein (Position: N174-P224). Human TIMP4 shares 92.2% amino acid (aa) sequence identity with both mouse and rat TIMP4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat Immunocytochemistry, 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human ELISA , 0.1-0.5μg/ml, Human, -
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 25503 MW
• UniProt ID: Q99727
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Metalloproteinase inhibitor 4;Tissue inhibitor of
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A549 cells using anti-TIMP4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of TIMP4 using anti-TIMP4 antibody.Lane 1:rat heart tissue;2:mouse heart tissue;3:HELA cell.

IF analysis of TIMP4 using anti-TIMP4 antibody. TIMP4 was detected in immunocytochemical section of HepG2 cells.

IHC analysis of TIMP4 using anti-TIMP4 antibody. TIMP4 was detected in paraffin-embedded section of rat kidney tissues.

IHC analysis of TIMP4 using anti-TIMP4 antibody. TIMP4 was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of TIMP4 using anti-TIMP4 antibody. TIMP4 was detected in paraffin-embedded section of human lung cancer tissues.