TIMP-2 Mouse Monoclonal Antibody

• Catalog Number: orb559157
• Category: Antibodies
• Description: TIMP-2 Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4C9
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: KLH conjugated synthetic peptide derived from human TIMP-2
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:20-100, IHC-F=1:20-100, IF=1:100-500, Flow-Cyt=1:20-50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 24 kDa
• Target: TIMP2
• UniProt ID: P16035
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Metalloproteinase inhibitor 2 precursor; Tissue in
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: K562. Primary Antibody (green line): Mouse Anti-TIMP-2 antibody (orb559157), dilution: 1:25, Secondary Antibody: Goat anti-mouse IgG-AF488, dilution: 1:400. Protocol, The cells were fixed with 2% PFA (10 min at room temperature) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (human kidney sections), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TIMP-2) Monoclonal Antibody, Unconjugated (orb559157) at 1:25 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Human HT-1080 cell lysates, Primary: Anti-TIMP-2 (orb559157) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 24 kD.

Sample: Lane 1: Human SW480 cell lysates, Primary: Anti-TIMP-2 (orb559157) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 24 kD.