TIM3 Antibody [10C10]

• Catalog Number: orb1240153
• Category: Antibodies
• Description: TIM3 Antibody [10C10]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 10C10
• Tested applications: ELISA, FC, ICC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1,k
• Immunogen: TIM-3 antibody was raised against the extracellular domain of human TIM-3
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 33 kDa Observed: 37 kDa
• Target: HAVCR2
• UniProt ID: Q8TDQ0
• NCBI: NP_116171
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TIM-3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Alternative names: TIM-3 Antibody: Hepatitis A virus cellular recepto
• Note: For research use only
• Application notes: TIM-3 antibody can be used for ELISA starting at 0.1 μg/mL. For Western blot start at 1 μg/mL. For Immunocytochemistry start at 1 μg/mL. For Immunofluorescence start at 10 μg/mL. For Immunohistochemistry start at 5 μg/mL. For Flow Cytometry start at 0.1 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of TIM-3 in overexpressing HEK293 cells TIM-3 antibody at (A) 0.25, (B) 0.5, and (C) 1 μg/ml

Immunocytochemistry of TIM-3 in transfected HEK293 cells with TIM-3 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.

Immunofluorescence of TIM-3 in transfected HEK293 cells with TIM-3 antibody at 10 μg/mL. Green: TIM-3 Antibody [10C10] (orb1240153) Blue: DAPI staining

Immunofluorescence of TIM-3 in human colon carcinoma tissue with TIM-3 antibody at 20 μg/mL. Green: TIM-3 Antibody [10C10] (orb1240153) Blue: DAPI staining

Immunohistochemistry of TIM-3 in human colon carcinoma tissue with TIM-3 antibody at 5 μg/mL.

Flow cytometry analysis of TIM-3 overexpressing HEK293 cells using TIM-3 antibody and control mouse IgG antibody at 0.1 μg/ml. Blue: Untransfected HEK293 cells. Yellow: TIM-3 overexpressing HEK293 cells.