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Catalog Number | orb1229866 |
---|---|
Category | Proteins |
Description | Tim-4 Recombinant Protein |
Tag | Fc Tag |
Form/Appearance | Lyophilized |
Purity | ≥98% (SDS-PAGE). Endotoxin level is less than 0.06EU/ μg protein (LAL test; Lonza). |
Target | Timd4 |
UniProt ID | O53649 |
Protein Sequence | The extracellular domain of mouse Tim-4 (aa 22-279) is fused to the N-terminus of the Fc region of human IgG1. |
Source | CHO cells |
Biological Activity | Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. |
NCBI | NP_848874 |
Storage | Stable for at least 1 year after receipt when stored at -20˚°C. Working aliquots are stable for up to 3 months when stored at -20˚°C. |
Buffer/Preservatives | Lyophilized from 0.2um-filtered solution in PBS. Reconstitute with 100 μl sterile water. Add 1X PBS to the desired protein concentration. |
Alternative names | TIM4, TIMD4, T Cell Immunoglobulin and Mucin Domai Read more... |
Note | For research use only |
Application notes | This recombinant proteins is for research use only. |
Expiration Date | 6 months from date of receipt. |
Greater than 85% as determined by SDS-PAGE. | |
37.4 kDa | |
E.coli |
Greater than 85% as determined by SDS-PAGE. | |
60.3 kDa | |
Mammalian cell |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
98.00% | |
31.55 kDa (predicted). Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
98.00% | |
32.43 kDa (predicted). Due to glycosylation, the protein migrates to 78-98 kDa based on Tris-Bis PAGE result. |