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Item 1 of 7
Item 1 of 7
TIM-3 Detection Set (Risk Free)
Catalog Number: orb1227436
| Catalog Number | orb1227436 |
|---|---|
| Category | Assays and Kits |
| Description | TIM-3 Detection Set (Risk Free) |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Tested applications | ELISA, FC, ICC, IF, IHC-P, WB |
| Reactivity | Human |
| Isotype | IgG1, IgG2b |
| Immunogen | TIM-3 antibody was raised against the extracellular domain of human TIM-3 |
| Concentration | Antibody 1 mg/mL |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| Storage | Antibodies can be stored at 4˚°C for three months and -20˚°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Buffer/Preservatives | PBS containing 0.02% sodium azide. |
| Note | For research use only |
| Expiration Date | Please enquire. |
Titration curve analysis of TIM-3 mAbs to detect recombinant TIM-3 in ELISA with orb1240153, orb1238174, orb1240159, orb1240157, orb1240154, orb1240156, and orb1240178 antibodies at decreasing concentrations.
Western blot analysis of TIM-3 in over expressing HEK293 cells using orb1240153, orb1240157, and orb1240154 antibodies at (A) 0.25 μg/ml, (B) 0.5 μg/ml, and (C) 1 μg/ml.
Immunocytochemistry of TIM-3 in over expressing HEK293 cells using (A) orb1240153, (B) orb1238174, (C) orb1240159, (D) orb1240157, (E) orb1240154, (F) orb1240156, (G) orb1240178, and (H) control mouse IgG antibody at 1 μg/ml.
Immunofluorescence of TIM-3 in over expressing HEK293 cells using (A) orb1240153, (B) orb1240159, (C) orb1240157, (D) orb1240154, (E) orb1240156, (F) orb1240178, and (G) control mouse IgG antibody at 10 μg/ml.
Immunofluorescence of TIM-3 in human colon carcinoma tissue using (A) orb1240153, (B) orb1240159, (C) orb1240157, (D) orb1240154, (E) orb1240156, (F) orb1240178, and (G) control mouse IgG antibody at 20 μg/ml.
Immunohistochemistry of TIM-3 in human colon carcinoma tissue using (A) orb1240153, (B) orb1240159, (C) orb1240157, (D) orb1240154, (E) orb1240156, (F) orb1240178, and (G) control mouse IgG antibody at 5 μg/ml.
Flow cytometry analysis of TIM-3 in over expressing HEK293 cells using (A) orb1240153, (B) orb1238174, (C) orb1240159, (D) orb1240157, (E) orb1240154, (F) orb1240156, (G) orb1240178, and (H) mouse IgG at 0.1 μg/ml. Blue: untransfected HEK293 cells. Yellow: TIM-3 over expressing HEK293 cells.
