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Catalog Number | orb758824 |
---|---|
Category | Antibodies |
Description | Rat monoclonal antibody to TIM-2 |
Species/Host | Rat |
Clonality | Monoclonal |
Clone Number | RMT2-14 |
Tested applications | Blocking, FA, FC, IHC, WB |
Reactivity | Mouse |
Isotype | IgG2a |
Immunogen | This antibody was raised by immunising Sprague Dawley rats with TIM-2-Ig consisting of the extracellular domain (aa 1-230 of mouse TIM-2) and the Fc portion of mouse IgG2a, emulsified in complete Freund's adjuvant and then fusing lymph node cells from the immunized rats with P3U1 myeloma cells. |
Concentration | 1 mg/ml |
Purity | Purified |
Conjugation | Unconjugated |
Target | TIM-2 |
UniProt ID | Q8R183 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | TIMD2; T Cell Immunoglobulin Mucin-2; T Cell immun Read more... |
Note | For research use only |
Application notes | This antibody has been used in various FACS analyses, for instance, to evaluate the expression of TIM family members on mouse mast cells (Nakae et al, 2007) and to block the H-ferritin binding to TIM-2/L5178Y cells (Kawamoto et al, 2011). This antibody has been used in FACS and immunohistochemistry analyses to show that TIM-2 is expressed on mouse fetal hepatocytes (Watanabe et al, 2007). This antibody has also been used in Western Blots and for blocking experiments in ferritin binding analysis, which suggests that Tim-2 is the H-ferritin receptor on oligodendrocytes (Todorich et al, 2008). In addition, this antibody has been used in collagen-induced arthritis (CIA) models to determine whether TIM-2 contributes to the development of T helper (Th) 1 or Th17 cells and joint inflammation (Kawamoto et al, 2011). |
Expiration Date | 12 months from date of receipt. |
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