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Catalog Number | orb1227441 |
---|---|
Category | Assays and Kits |
Description | TIGIT Detection Set (Risk Free) |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | ELISA, FC, ICC, IF, IHC-P |
Reactivity | Human |
Isotype | IgG1, IgG2a, IgG2b |
Immunogen | TIGIT antibodies were raised against the extracellular domain of human TIGIT. |
Concentration | Antibody 1 mg/mL |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Storage | Antibodies can be stored at 4˚°C for three months and -20˚°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | PBS containing 0.02% sodium azide. |
Note | For research use only |
Expiration Date | Please enquire. |
Titration curve analysis of TIGIT mAbs to detect recombinant TIGIT in ELISA with orb1240147, orb1240136, orb1240137, orb1240140, orb1240146 and orb1240115 antibodies at decreasing concentrations.
Western blot analysis of TIGIT in over expressing HEK293 cells using orb1240137, orb1240140, and orb1240146 antibodies at (A) 0.25 μg/ml, (B) 0.5 μg/ml, and (C) 1 μg/ml.
Immunocytochemistry of TIGIT in over expressing HEK293 cells using (A) orb1240147, (B) orb1240136, (C) orb1240137, (D) orb1240140, (E) orb1240146, (F) orb1240115, and (G) control mouse IgG antibody at 1 μg/ml.
Immunofluorescence of TIGIT in over expressing HEK293 cells using (A) orb1240147, (B) orb1240136, (C) orb1240137, (D) orb1240140, (E) orb1240146, (F) orb1240115, and (G) control mouse IgG antibody at 1 μg/ml.
Immunofluorescence of TIGIT in human stomach carcinoma tissue using (A) orb1240147, (B) orb1240136, (C) orb1240137, (D) orb1240140, (E) orb1240146, (F) orb1240115, and (G) control mouse IgG antibody at 5 μg/ml.
Immunohistochemistry of TIGIT in human stomach carcinoma tissue (A) orb1240147, (B) orb1240136, (C) orb1240137, (D) orb1240140, (E) orb1240146, (F) orb1240115, and (G) control mouse IgG antibody at 2 μg/ml.
Flow cytometry analysis of TIGIT over expressing HEK293 cells using (A) orb1240147, (B) orb1240136, (C) orb1240137, (D) orb1240140, (E) orb1240146, (F) orb1240115, and (G) control mouse IgG antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.