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Catalog Number | orb97097 |
---|---|
Category | Antibodies |
Description | Tight junction protein ZO-2 TJP2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Predicted Reactivity | Hamster |
Reactivity | Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse TJP2 (1149-1167aa AHSKRGYYSQPSRYRDTEL), identical to the related rat sequence. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Mouse, Rat |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 131280 MW |
UniProt ID | Q9Z0U1 |
Sensitivity | > 5000 cells |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Tight junction protein ZO-2;Tight junction protein Read more... |
Note | For research use only |
Application notes | WB: The detection limit for TJP2 is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Lane 1: Rat Brain Tissue Lysate, Lane 2: PC-12 Cell Lysate, Lane 3: Mouse Brain Tissue Lysate, Lane 4: HEPA Cell Lysate using TJP2 antibody
Immunohistochemical analysis of formalin-fixed paraffin embedded Mouse Liver Tissue using TJP2 antibody
FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Bovine, Canine, Equine, Gallus, Guinea pig, Rabbit, Sheep, Zebrafish | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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