TIF1 gamma/TRIM33 Antibody

• Catalog Number: orb334568
• Category: Antibodies
• Description: TIF1 gamma/TRIM33 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Hamster, Monkey, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human TIF1 gamma recombinant protein (Position: M1001-K1127). Human TIF1 gamma shares 96.1% amino acid (aa) sequence identity with mouse TIF1 gamma.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 122533 MW
• UniProt ID: Q9UPN9
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: E3 ubiquitin-protein ligase TRIM33;6.3.2.-;Ectoder
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U87 cells using anti-TIF1 gamma antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of A431 cells using anti-TIF1 gamma antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of TIF1 gamma using anti-TIF1 gamma antibody.Lane 1:human K562 cell; 2:human Jurkat cell; 3:rat brain tissue; 4:mouse brain tissue.

IF analysis of TIF1 gamma using anti-TIF1 gamma antibody. TIF1 gamma was detected in immunocytochemical section of A431 cells.

IHC analysis of TIF1 gamma using anti-TIF1 gamma antibody. TIF1 gamma was detected in a paraffin-embedded section of human lung adenocarcinoma tissue.

IHC analysis of TIF1 gamma using anti-TIF1 gamma antibody. TIF1 gamma was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue.