TIF-IA Antibody

• Catalog Number: orb1525518
• Category: Antibodies
• Description: TIF-IA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: between 601 and 651
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: TIF-IA
• UniProt ID: Q9NYV6
• NCBI: NP_060897.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: A-270G1.2; TIF-IA; transcription initiation factor
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human TIF-IA by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-TIF-IA antibody orb1525518 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. TIF-IA was also immunoprecipitated by rabbit anti-TIF-IA antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).