TIA-1 Antibody / T-Cell Intracellular Antigen-1

• Catalog Number: orb2636575
• Category: Antibodies
• Description: The T cell intracellular antigen 1 (TIA 1) is a 17 kDa cytoplasmic granule associated protein also designated as GMP 17, for granule membrane protein of 17 kDa. The GMP 17/TIA 1 molecule is expressed in cells possessing cytolytic potential and could be involved in the signaling cascade of Fas (CD95) mediated apoptosis. Within hematopoietic cell lines, the TIA1/1352R monoclonal antibody reacts with about 90% of CD16+, 50 60% of CD8+, and less than 10% of CD4+ normal peripheral blood lymphocytes. It reacts with almost all monocytes and granulocytes. This antibody also reacts with CD4+ activated T cell clones, activated NK cell clones, and Con activated thymocytes, but not with B lymphocytes or B cell lines. TIA1 antibody labels cytotoxic T cells and natural killer cells (NK cells). It is also expressed in T-cell lymphoma, large granular lymphocyte (LGL) leukemia and hairy cell leukemia. TIA1 expression in T-cell malignancies may help in differentiating LGL leukemia (high expression) from T-cell lymphocytosis and other T-cell diseases (low expression). TIA1 may also be used to label tumor-infiltrating lymphocytes in the study of immune response to malignancies.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: TIA1/1352R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: Human bone marrow malignant cells from a non B, non T acute leukemia were used as the immunogen for the recombinant TIA-1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant TIA-1 antibody is available for research use only.
• UniProt ID: P31483
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant TIA-1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human lymph node with recombinant TIA-1 antibody (clone TIA/1352R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human spleen tissue with recombinant TIA-1 antibody (clone TIA/1352R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with recombinant TIA-1 antibody (clone TIA/1352R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human brain tissue with recombinant TIA-1 antibody (clone TIA1/1352R) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.