Thyroid Stimulating Hormone Receptor / TSHR Antibody (alpha chain)

• Catalog Number: orb2640272
• Category: Antibodies
• Description: Thyroid-stimulating hormone (TSH, also known as thyrotropin) is a glycoprotein involved in the control of thyroid structure and metabolism, which stimulates the release of the thyroid hormones. TSH is regulated by thyroid hormone (T3) and various retinoid compounds. TSH binds to the thyroid-stimulating hormone receptor (TSHR), which is cleaved into two subunits, A and B, and plays a major role in regulating thyroid function. The third cytoplasmic loop of TSHR has been identified as critical for its role in regulating inositol phosphate and cAMP formation. In Graves disease, an autoimmune disorder, TSHR is activated by autoantibodies, which may be stimulated by the cleavage of the A and B subunits.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: Recombinant human TSH Receptor alpha chain protein was used as the immunogen for this TSHR antibody.
• UniProt ID: P16473
• Tested applications: ELISA, IHC-P
• Dilution range: ELISA: 2-4ug/ml (order BSA/azide-free format),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TSHR antibody to be titered up or down for optimal performance.
• Antibody Type: Primary Antibody
• Clone Number: TSHRA/1402
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This TSHR antibody is available for research use only.
• Note: For research use only

IHC testing of FFPE human thyroid carcinoma with TSHR antibody (clone TSHRA/1402). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, or 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE Analysis of Purified, BSA-Free TSHR Antibody (clone TSHRA/1402). Confirmation of Integrity and Purity of the Antibody.